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Article

Improved Incorporation of Noncanonical Amino Acids by an Engineered tRNA(Tyr) Suppressor.

Biochemistry - 26 Jan 2016

Rauch Benjamin J, Porter Joseph J, Mehl Ryan A, Perona John J

Abstract excerpt

The Methanocaldcoccus jannaschii tyrosyl-tRNA synthetase (TyrRS):tRNA(Tyr) cognate pair has been used to incorporate a large number of noncanonical amino acids (ncAAs) into recombinant proteins in Escherichia coli. However, the structural elements of the suppressor tRNA(Tyr) used in these experiments have not been examined for optimal performance. Here, we evaluate the steady-state kinetic parameters of wild-type...

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Identifiers

PMID: 26694948
DOI: 10.1021/acs.biochem.5b01185
PMCID: PMC4959848

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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