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Article

Evolving Escherichia coli to use a tRNA with a non-canonical fold as an adaptor of the genetic code.

Nucleic acids research - 11 Nov 2024

Edelmann Martin P, Couperus Sietse, Rodríguez-Robles Emilio, Rivollier Julie, Roberts Tania M, Panke Sven, Marlière Philippe

Abstract excerpt

All known bacterial tRNAs adopt the canonical cloverleaf 2D and L-shaped 3D structures. We aimed to explore whether alternative tRNA structures could be introduced in bacterial translation. To this end, we crafted a vitamin-based genetic system to evolve Escherichia coli toward activity of structurally non-canonical tRNAs. The system reliably couples (escape frequency <10-12) growth with the activities of a novel...

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Identifiers

PMID: 39315692
DOI: 10.1093/nar/gkae806
PMCID: PMC11551756

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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