Article
Suppression of a double missense mutation by a mutant tRNA(Phe) in Escherichia coli.
Nucleic acids research - 25 Feb 1991
Pages D, Hijazi K, Murgola E J, Finelli J, Buckingham R H
Abstract excerpt
We report here the isolation of a mutant tRNAPhe that suppresses a double missense auxotrophic mutation in trpA of Escherichia coli, trpA218. The doubly mutant protein product differs from wild-type TrpA by the replacements of Phe22 by Leu and Gly211 by Ser. A partial revertant TrpA phenotype can be obtained from trpA218 by changing either Leu22 back to Phe or Ser211 back to Gly. Translational suppressors were...
Topics
- Base Sequence
- Codon
- Escherichia coli
- Genes, Bacterial
- Genes, Suppressor
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Polymerase Chain Reaction
- RNA, Transfer, Phe