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Article

Selection for active E. coli tRNA(Phe) variants from a randomized library using two proteins.

The EMBO journal - 1 Jul 1993

Peterson E T, Blank J, Sprinzl M, Uhlenbeck O C

Abstract excerpt

In vitro selection was used to isolate active Escherichia coli tRNA(Phe) variants from randomized libraries. Functional tRNAs were first selected by multiple rounds of binding to Escherichia coli phenylalanyl-tRNA synthetase. These variants were then aminoacylated and selected for affinity to elongation factor-Tu. By randomizing potential recognition nucleotides, the importance of residues U20, G34, A35, A36 and...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 7687542
DOI: 10.1002/j.1460-2075.1993.tb05958.x
PMCID: PMC413551

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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