Selection for active E. coli tRNA(Phe) variants from a randomized library using two proteins.

doi:10.1002/j.1460-2075.1993.tb05958.x

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Article

Selection for active E. coli tRNA(Phe) variants from a randomized library using two proteins.

The EMBO journal - 1 Jul 1993

Peterson E T, Blank J, Sprinzl M, Uhlenbeck O C

Abstract excerpt

In vitro selection was used to isolate active Escherichia coli tRNA(Phe) variants from randomized libraries. Functional tRNAs were first selected by multiple rounds of binding to Escherichia coli phenylalanyl-tRNA synthetase. These variants were then aminoacylated and selected for affinity to elo...

Topics

Base Sequence
Escherichia coli
Gene Library
Genetic Variation
Molecular Sequence Data
Nucleic Acid Conformation
Peptide Elongation Factor Tu
Phenylalanine-tRNA Ligase
RNA, Bacterial
RNA, Transfer, Phe

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