Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens.

Transgenic research - 1 Apr 2024

Calbry Julie, Goudounet Guillaume, Charlot Florence, Guyon-Debast Anouchka, Perroud Pierre-François, Nogué Fabien

Abstract excerpt

Genome editing via CRISPR/Cas has enabled targeted genetic modifications in various species, including plants. The requirement for specific protospacer-adjacent motifs (PAMs) near the target gene, as seen with Cas nucleases like SpCas9, limits its application. PAMless SpCas9 variants, designed with a relaxed PAM requirement, have widened targeting options. However, these so-call PAMless SpCas9 still show...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bryopsida
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Cas Systems

Share to Gene Editing

Share to Genome, Plant

Share to Mutation

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 38573428
DOI: 10.1007/s11248-024-00381-1
PMCID: PMC11021247

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens.PMID 38573428Read ↗

Genome editing mediated by SpCas9 variants with broad non-canonical PAM compatibility in plants.Semantic proximity Read ↗

Expanding base editing scope to near-PAMless with engineered CRISPR/Cas9 variants in plantsSemantic proximity Read ↗

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.Semantic proximity Read ↗

Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor.Semantic proximity Read ↗

Expanding PAM recognition and enhancing base editing activity of Cas9 variants with non-PI domain mutations derived from xCas9.Semantic proximity Read ↗

Engineered CRISPR-Cas9 nucleases with altered PAM specificities.Semantic proximity Read ↗

PAM-Flexible Genome Editing with an Engineered Chimeric Cas9Semantic proximity Read ↗

SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition.Semantic proximity Read ↗

Induced mutation and epigenetics modification in plants for crop improvement by targeting CRISPR/Cas9 technology.Semantic proximity Read ↗

Diverse noncanonical PAMs recognized by SpCas9 in human cellsSemantic proximity Read ↗

Increasing the Activity of the High-Fidelity SpyCas9 Form in Yeast by Directed Mutagenesis of the PAM-Interacting Domain.Semantic proximity Read ↗

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.