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Tunable Control of an Escherichia coli Expression System for the Overproduction of Membrane Proteins by Titrated Expression of a Mutant lac Repressor.

ACS synthetic biology - 15 Sept 2017

Kim Seong Keun, Lee Dae-Hee, Kim Oh Cheol, Kim Jihyun F, Yoon Sung Ho

Abstract excerpt

Most inducible expression systems suffer from growth defects, leaky basal induction, and inhomogeneous expression levels within a host cell population. These difficulties are most prominent with the overproduction of membrane proteins that are toxic to host cells. Here, we developed an Escherichia coli inducible expression system for membrane protein production based on titrated expression of a mutant lac...

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Identifiers

PMID: 28524655
DOI: 10.1021/acssynbio.7b00102

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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