Rapid screening of point mutations by mismatch amplification mutation assay PCR.

Abstract excerpt

Metabolic engineering frequently makes use of point mutation and saturation mutation library creation. At present, sequencing is the only reliable and direct technique to detect point mutation and screen saturation mutation library. In this study, mismatch amplification mutation assay (MAMA) PCR was used to detect point mutation and screen saturation mutation library. In order to fine-tune the expression of odhA...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Corynebacterium glutamicum