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Article

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors.

Nature biotechnology - 1 Jul 2020

Li Chao, Zhang Rui, Meng Xiangbing, Chen Sha, Zong Yuan, Lu Chunju, Qiu Jin-Long, Chen Yu-Hang, Li Jiayang, Gao Caixia

Abstract excerpt

Targeted saturation mutagenesis of crop genes could be applied to produce genetic variants with improved agronomic performance. However, tools for directed evolution of plant genes, such as error-prone PCR or DNA shuffling, are limited1. We engineered five saturated targeted endogenous mutagenesis editors (STEMEs) that can generate de novo mutations and facilitate directed evolution of plant genes. In rice...

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Identifiers

PMID: 31932727
DOI: 10.1038/s41587-019-0393-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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