Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Simple-MSSM: a simple and efficient method for simultaneous multi-site saturation mutagenesis.

Biotechnology letters - 1 Apr 2017

Cheng Feng, Xu Jian-Miao, Xiang Chao, Liu Zhi-Qiang, Zhao Li-Qing, Zheng Yu-Guo

Abstract excerpt

OBJECTIVE: To develop a practically simple and robust multi-site saturation mutagenesis (MSSM) method that enables simultaneously recombination of amino acid positions for focused mutant library generation. RESULTS: A general restriction enzyme-free and ligase-free MSSM method (Simple-MSSM) based on prolonged overlap extension PCR (POE-PCR) and Simple Cloning techniques. As a proof of principle of Simple-MSSM,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acids
Share to Codon
Share to Green Fluorescent Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutagenesis

Share to Mutagenesis, Site-Directed

Share to Oligonucleotides

Share to Protein Engineering

Share to Transformation, Genetic

Source

Identifiers

PMID: 28044225
DOI: 10.1007/s10529-016-2278-x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Simple-MSSM: a simple and efficient method for simultaneous multi-site saturation mutagenesis.PMID 28044225Read ↗

CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes.Semantic proximity Read ↗

Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.Semantic proximity Read ↗

Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.Semantic proximity Read ↗

Codon compression algorithms for saturation mutagenesis.Semantic proximity Read ↗

Plasmid-based one-pot saturation mutagenesis.Semantic proximity Read ↗

MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR.Semantic proximity Read ↗

An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening.Semantic proximity Read ↗

PCR-based strategy for construction of multi-site-saturation mutagenic expression library.Semantic proximity Read ↗

Recent advances in targeted mutagenesis to expedite the evolution of biological systems.Semantic proximity Read ↗

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids.Semantic proximity Read ↗

Amber codon-mediated expanded saturation mutagenesis of proteins using a cell-free translation system.Semantic proximity Read ↗

Rapid gene splicing and multi-sited mutagenesis by one-step overlap extension polymerase chain reaction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.