Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9.

STAR protocols - 15 Dec 2023

Zhu Yicheng, Tahara Stanley M, Tsukamoto Hidekazu, Machida Keigo

Abstract excerpt

We detail procedures for generating a humanized mouse model of hepatocellular carcinoma (HCC) recapitulating genetic mutations associated with metabolic liver diseases (MLD). We humanized liver parenchymal, non-parenchymal, and hematopoietic cells. We employed CRISPR-Cas9-based ARID1A knockout and constitutively active CTNNB1 knockin combined with an alcohol Western diet to generate cancer-driver mutations...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Carcinoma, Hepatocellular
Share to CRISPR-Cas Systems
Share to Disease Models, Animal

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Liver Neoplasms

Share to Mutation

Source

Open in PubMed Open full text

Identifiers

PMID: 38103196
DOI: 10.1016/j.xpro.2023.102389
PMCID: PMC10751556

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9.PMID 38103196Read ↗

Genetically Engineered Mouse Models for Liver CancerSemantic proximity Read ↗

There Is Something Fishy About Liver Cancer: Zebrafish Models of Hepatocellular CarcinomaSemantic proximity Read ↗

CRISPR activation screening in a mouse model for drivers of hepatocellular carcinoma growth and metastasisSemantic proximity Read ↗

Integrative genomic analysis of mouse and human hepatocellular carcinomaSemantic proximity Read ↗

Zebrafish as a disease model for studying human hepatocellular carcinoma.Semantic proximity Read ↗

Human-correlated genetic HCC models identify combination therapy for precision medicineSemantic proximity Read ↗

A new chemical carcinogen and Western diet protocol to reliably induce advanced hepatocellular carcinoma in male and female miceSemantic proximity Read ↗

Genome-wide mapping of oncogenic pathways and genetic modifiers of chemotherapy using a high-risk hepatoblastoma genetic modelSemantic proximity Read ↗

Murine Liver Organoids as a Genetically Flexible System to Study Liver Cancer In Vivo and In VitroSemantic proximity Read ↗

CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates That the Dnajb1-Prkaca Gene Fusion Is Sufficient to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma.Semantic proximity Read ↗

Human-correlated genetic models identify precision therapy for liver cancer.Semantic proximity Read ↗

Deleting <i>in vivo</i> β-catenin degradation domain in mouse hepatocytes drives hepatocellular carcinoma or hepatoblastoma-like tumorsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.