Article
Deletion of the Candida albicans TLO gene family using CRISPR-Cas9 mutagenesis allows characterisation of functional differences in α-, β- and γ- TLO gene function.
PLoS genetics - 1 Dec 2023
Fletcher Jessica, O'Connor-Moneley James, Frawley Dean, Flanagan Peter R, Alaalm Leenah, Menendez-Manjon Pilar, Estevez Samuel Vega, Hendricks Shane, Woodruff Andrew L, Buscaino Alessia, Anderson Matthew Z, Sullivan Derek J, Moran Gary P
Abstract excerpt
The Candida albicans genome contains between ten and fifteen distinct TLO genes that all encode a Med2 subunit of Mediator. In order to investigate the biological role of Med2/Tlo in C. albicans we deleted all fourteen TLO genes using CRISPR-Cas9 mutagenesis. ChIP-seq analysis showed that RNAP II localized to 55% fewer genes in the tloΔ mutant strain compared to the parent, while RNA-seq analysis showed that the...
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