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Article

A high-throughput protocol for deamination of long single-stranded DNA and oligo pools containing complex sequences.

STAR protocols - 15 Dec 2023

Wang Yanyan, Zhang Senxin, Zheng Xiaoqi, Yeap Leng-Siew, Meng Fei-Long

Abstract excerpt

Cytidine deaminases as DNA mutators play important roles in immunity and genome stability. Here, we present a high-throughput protocol for deamination of long single-stranded (ss) DNA or oligo pools containing complex sequences. We describe steps for the preparation of both enzyme (activation-induced deaminase, AID) and ssDNA substrates, the deamination reaction, uracil-friendly amplification, and data analysis....

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Identifiers

PMID: 37742176
DOI: 10.1016/j.xpro.2023.102602
PMCID: PMC10522977

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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