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Article

Mapping temperature-sensitive mutations at a genome scale to engineer growth switches in Escherichia coli.

Molecular systems biology - 12 Oct 2023

Schramm Thorben, Lubrano Paul, Pahl Vanessa, Stadelmann Amelie, Verhülsdonk Andreas, Link Hannes

Abstract excerpt

Temperature-sensitive (TS) mutants are a unique tool to perturb and engineer cellular systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each with a single amino acid change in one of 346 essential proteins. 1,269 of these mutants showed temperature-sensitive growth in a time-resolved competition assay. We reconstructed 94 TS mutants and measured their metabolism under growth...

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Identifiers

PMID: 37642940
DOI: 10.15252/msb.202311596
PMCID: PMC10568205

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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