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Article

Strategy for directing combinatorial genome engineering in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America - 26 Jun 2012

Sandoval Nicholas R, Kim Jaoon Y H, Glebes Tirzah Y, Reeder Philippa J, Aucoin Hanna R, Warner Joseph R, Gill Ryan T

Abstract excerpt

We describe a directed genome-engineering approach that combines genome-wide methods for mapping genes to traits [Warner JR, Reeder PJ, Karimpour-Fard A, Woodruff LBA, Gill RT (2010) Nat Biotechnol 28:856-862] with strategies for rapidly creating combinatorial ribosomal binding site (RBS) mutation libraries containing billions of targeted modifications [Wang HH, et al. (2009) Nature 460:894-898]. This approach...

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PMID: 22689973
DOI: 10.1073/pnas.1206299109
PMCID: PMC3387050

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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