Article
Metabolic response to point mutations reveals principles of modulation of in vivo enzyme activity and phenotype.
Molecular systems biology - 1 Jun 2021
Bhattacharyya Sanchari, Bershtein Shimon, Adkar Bharat V, Woodard Jaie, Shakhnovich Eugene I
Abstract excerpt
The relationship between sequence variation and phenotype is poorly understood. Here, we use metabolomic analysis to elucidate the molecular mechanism underlying the filamentous phenotype of E. coli strains that carry destabilizing mutations in dihydrofolate reductase (DHFR). We find that partial loss of DHFR activity causes reversible filamentation despite SOS response indicative of DNA damage, in contrast to...
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