Article
Massively parallel enrichment of low-frequency alleles enables duplex sequencing at low depth.
Nature biomedical engineering - 1 Mar 2022
Gydush Gregory, Nguyen Erica, Bae Jin H, Blewett Timothy, Rhoades Justin, Reed Sarah C, Shea Douglas, Xiong Kan, Liu Ruolin, Yu Fangyan, Leong Ka Wai, Choudhury Atish D, Stover Daniel G, Tolaney Sara M, Krop Ian E, Christopher Love J, Parsons Heather A, Mike Makrigiorgos G, Golub Todd R, Adalsteinsson Viktor A
Abstract excerpt
Assaying for large numbers of low-frequency mutations requires sequencing at extremely high depth and accuracy. Increasing sequencing depth aids the detection of low-frequency mutations yet limits the number of loci that can be simultaneously probed. Here we report a method for the accurate tracking of thousands of distinct mutations that requires substantially fewer reads per locus than conventional...
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