Article
Unbiased whole genome detection of ultrarare off-target mutations in genome-edited cell populations by HiFi sequencing.
Environmental and molecular mutagenesis - 1 Aug 2023
Miranda Jaime A, Fenner Kristina, McKinzie Page B, Dobrovolsky Vasily N, Revollo Javier R
Abstract excerpt
DNA base editors (BEs) composed of a nuclease-deficient Cas9 fused to a DNA-modifying enzyme can achieve on-target mutagenesis without creating double-strand DNA breaks (DSBs). As a result, BEs generate far less DNA damage than traditional nuclease-proficient Cas9 systems, which do rely on the creation of DSBs to achieve on-target mutagenesis. The inability of BEs to create DSBs makes the detection of their...
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