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Article

A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spike.

Cell - 16 Mar 2023

Dadonaite Bernadeta, Crawford Katharine H D, Radford Caelan E, Farrell Ariana G, Yu Timothy C, Hannon William W, Zhou Panpan, Andrabi Raiees, Burton Dennis R, Liu Lihong, Ho David D, Chu Helen Y, Neher Richard A, Bloom Jesse D

Abstract excerpt

A major challenge in understanding SARS-CoV-2 evolution is interpreting the antigenic and functional effects of emerging mutations in the viral spike protein. Here, we describe a deep mutational scanning platform based on non-replicative pseudotyped lentiviruses that directly quantifies how large numbers of spike mutations impact antibody neutralization and pseudovirus infection. We apply this platform to produce...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 36868218
DOI: 10.1016/j.cell.2023.02.001
PMCID: PMC9922669

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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