Article
Ligand-specific changes in conformational flexibility mediate long-range allostery in the lac repressor.
Nature communications - 2 Mar 2023
Glasgow Anum, Hobbs Helen T, Perry Zion R, Wells Malcolm L, Marqusee Susan, Kortemme Tanja
Abstract excerpt
Biological regulation ubiquitously depends on protein allostery, but the regulatory mechanisms are incompletely understood, especially in proteins that undergo ligand-induced allostery with few structural changes. Here we used hydrogen-deuterium exchange with mass spectrometry (HDX/MS) to map allosteric effects in a paradigm ligand-responsive transcription factor, the lac repressor (LacI), in different functional...
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