Article
High resolution melting analysis of almond SNPs derived from ESTs.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Dec 2008
Wu Shu-Biao, Wirthensohn Michelle G, Hunt Peter, Gibson John P, Sedgley Margaret
Abstract excerpt
High resolution melting curve (HRM) is a recent advance for the detection of SNPs. The technique measures temperature induced strand separation of short PCR amplicons, and is able to detect variation as small as one base difference between samples. It has been applied to the analysis and scan of mutations in the genes causing human diseases. In plant species, the use of this approach is limited. We applied HRM...
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