Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A simplified procedure for antibody engineering by yeast surface display: Coupling display levels and target binding by ribosomal skipping

23 Nov 2016

Abstract excerpt

Yeast surface display is a valuable, widely used method for protein engineering. However, current yeast display applications rely on the staining of epitope tags in order to verify full-length presentation of the protein of interest on the cell surface. We aimed at developing a modified yeast display approach that relies on ribosomal skipping, thereby enabling the translation of two proteins from one open reading...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Monoclonal and Polyclonal Antibodies Research
Share to Transgenic Plants and Applications
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 27878965
DOI: 10.1002/biot.201600454

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A simplified procedure for antibody engineering by yeast surface display: Coupling display levels and target binding by ribosomal skippingPMID 27878965Read ↗

Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian CellsSemantic proximity Read ↗

Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface displaySemantic proximity Read ↗

PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein RegulatorsSemantic proximity Read ↗

Yeast Surface Display System: Strategies for Improvement and Biotechnological ApplicationsSemantic proximity Read ↗

Yeast display of antibody fragments: a discovery and characterization platformSemantic proximity Read ↗

Rapidly Inducible Yeast Surface Display for Antibody Evolution with OrthoRepSemantic proximity Read ↗

Epitope-Specific Binder Design by Yeast Surface Display.Semantic proximity Read ↗

Expanding and improving nanobody repertoires using a yeast display method: Targeting SARS-CoV-2Semantic proximity Read ↗

Therapeutic antibody engineering by high efficiency cell screeningSemantic proximity Read ↗

Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments.Semantic proximity Read ↗

Facile generation of antibody heavy and light chain diversities for yeast surface display by Golden Gate CloningSemantic proximity Read ↗

A fluorescent protein scaffold for presenting structurally constrained peptides provides an effective screening system to identify high affinity target-binding peptides.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.