Article
DNA extraction from clotted blood in genotyping quality.
BioTechniques - 1 Jan 2023
Stanzick Kira J, Simon Josef, Zimmermann Martina E, Schachtner Michael, Peterhoff David, Niller Hans-Helmut, Überla Klaus, Wagner Ralf, Heid Iris M, Stark Klaus J
Abstract excerpt
DNA extraction from frozen blood clots is challenging. Here, the authors applied QIAGEN Clotspin Baskets and the Gentra Puregene Blood Kit for DNA extraction to cellular fraction of 5.5 ml whole blood without anticoagulating additives. The amount and quality of extracted DNA were assessed via spectrophotometer and gel electrophoresis. Results from array-based genotyping were analyzed. All steps were compared with...
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