Article
An alternate method for DNA and RNA extraction from clotted blood.
Genetics and molecular research : GMR - 4 Feb 2013
Zakaria Z, Umi S H, Mokhtar S S, Mokhtar U, Zaiharina M Z, Aziz A T A, Hoh B P
Abstract excerpt
We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit was used for DNA extraction. Extracted DNA and RNA were adequate for gene expression analysis and copy number...
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