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Article

Deep mutational scan of a drug efflux pump reveals its structure-function landscape.

Nature chemical biology - 1 Apr 2023

Meier Gianmarco, Thavarasah Sujani, Ehrenbolger Kai, Hutter Cedric A J, Hürlimann Lea M, Barandun Jonas, Seeger Markus A

Abstract excerpt

Drug efflux is a common resistance mechanism found in bacteria and cancer cells, but studies providing comprehensive functional insights are scarce. In this study, we performed deep mutational scanning (DMS) on the bacterial ABC transporter EfrCD to determine the drug efflux activity profile of more than 1,430 single variants. These systematic measurements revealed that the introduction of negative charges at...

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Identifiers

PMID: 36443574
DOI: 10.1038/s41589-022-01205-1
PMCID: PMC7615509

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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