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Article

Degradation-Suppressed Cocoonase for Investigating the Propeptide-Mediated Activation Mechanism.

Molecules (Basel, Switzerland) - 20 Nov 2022

Sakata Nana, Ogata Ayumi, Takegawa Mai, Murakami Yuri, Nishimura Misaki, Miyazawa Mitsuhiro, Hagiwara Teruki, Shimamoto Shigeru, Hidaka Yuji

Abstract excerpt

Cocoonase is folded in the form of a zymogen precursor protein (prococoonase) with the assistance of the propeptide region. To investigate the role of the propeptide sequence on the disulfide-coupled folding of cocoonase and prococoonase, the amino acid residues at the degradation sites during the refolding and auto-processing reactions were determined by mass spectrometric analyses and were mutated to suppress...

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Identifiers

PMID: 36432163
DOI: 10.3390/molecules27228063
PMCID: PMC9693254

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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