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Mutational Analysis of Substrate Recognition in Trypsin-like Protease Cocoonase: Protein Memory Induced by Alterations in Substrate-Binding Site.

Molecules (Basel, Switzerland) - 20 Nov 2024

Sakata Nana, Shimamoto Shigeru, Murakami Yuri, Ashida Orika, Takei Toshiki, Miyazawa Mitsuhiro, Hidaka Yuji

Abstract excerpt

To investigate the substrate recognition mechanism of trypsin-like protease cocoonase (CCN), mutational analyses were conducted at key substrate recognition sites, Asp187 and Ser188, and their effects on substrate specificity and enzymatic activity were evaluated. Mutants with the Asp187 substitution exhibited a significant reduction in catalytic activity compared with the wild-type enzyme, whereas the Ser188...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 39598863
DOI: 10.3390/molecules29225476
PMCID: PMC11597625

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