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Article

Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase.

Protein science : a publication of the Protein Society - 1 Jul 2004

Liao You-Di, Jeng Jen-Chong, Wang Chiu-Feng, Wang Sui-Chi, Chang Shu-Ting

Abstract excerpt

The removal of N-terminal translation initiator Met by methionine aminopeptidase (MetAP) is often crucial for the function and stability of proteins. On the basis of crystal structure and sequence alignment of MetAPs, we have engineered Escherichia coli MetAP by the mutation of three residues, Y1...

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Identifiers

PMID: 15215523
DOI: 10.1110/ps.04679104
PMCID: PMC2279930

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-15215523-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

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