Article
Taqman-MGB FQ-PCR for Human GPIIIa, GP1BA and PEAR1 SNPs.
Clinical laboratory - 1 Oct 2022
Cheng Hongxia, Liu Yuhua, Fu Min, Liu Jingjing, Chen Gang
Abstract excerpt
BACKGROUND: A reliable operation-convenient Taqman-MGB probe fluorescence quantity polymerase chain reaction (FQ-PCR) for detection human GPIIIa, GP1BA, and PEAR1 polymorphism were designed, and the performances were assessed. METHODS: Four sets of probes and primers for target alleles included rs5918 (176T>C), rs6065 (5792C>T), rs1204133 (2266G>A), and β-actin were designed, the reaction systems were optimized....
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