Article
Screening, Confirmation and Validation of Mismatch AS F-Primers for Hypertension-Related Genotyping.
Studies in health technology and informatics - 23 Nov 2023
Yang Ping, Lan Wei, Su Quanlin, Li Chunyan, Zheng Jie, Zhang Jing, Hu Yue, Lan Wenjun
Abstract excerpt
In this study, screening, confirmation and validation of mismatch allele-specific (AS) forward (F)-primers are executed to establish a quadruplex amplification analysis (real-time PCR) for discrimination of CYP2D6*10, ADRB1, NPPA and CYP3A5*3 genotypes associated with hypertensive pharmacogenomics. To significantly distinguish heterozygote and homozygote, ΔCq (differences in threshold cycles between the wild-type...
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