Article
Multicolor real-time polymerase chain reaction genotyping of six human platelet antigens using displacing probes.
Transfusion - 1 Sept 2007
Ruan Li, Pei Bin, Li Qingge
Abstract excerpt
BACKGROUND: Several genotyping methods for six clinically relevant human platelet antigens (HPAs) have been reported. A four-color real-time polymerase chain reaction (PCR) method using displacing probes for genotyping of the six HPAs is described. STUDY DESIGN AND METHODS: Primers and four differently fluorophor-labeled displacing probes were designed and synthesized to detect single-nucleotide polymorphisms...
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