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Whole Blood PCR Amplification with Pfu DNA Polymerase and Its Application in Single-Nucleotide Polymorphism Analysis.

Genetic testing and molecular biomarkers - 1 Nov 2015

Liu Er-Ping, Wang Yan, He Xiao-Hui, Guan Jun-Jie, Wang Jin, Qin Zheng-Hong, Sun Wan-Ping

Abstract excerpt

AIMS: Point-of-care genetic analysis may require polymerase chain reaction (PCR) to be carried out on whole blood. However, human blood contains natural inhibitors of PCR such as hemoglobin, immunoglobulin G, lactoferrin, and proteases, as well as anticoagulant agents, including EDTA and heparin that can reduce whole blood PCR efficiency. Our purpose was to develop a highly specific, direct whole blood...

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PMID: 26360116
DOI: 10.1089/gtmb.2015.0018

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VKORC1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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