Article
An Improved PCR-RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene.
Journal of clinical laboratory analysis - 1 Nov 2016
Feng Xiaolei, Wang Sihua, Duan Xiaoran, Li Chunyang, Yan Zhen, Feng Feifei, Yu Songcheng, Wu Yongjun, Wang Wei
Abstract excerpt
BACKGROUND: In recent research, it has been shown that rs2289487 within the PLIN1 gene has different variants that have been associated with obesity, type 2 diabetes, and other diseases. However, the isochizomers such as the BsmI enzyme required for detection of this polymorphism through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method are expensive. In this study, we aimed to...
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