Article
A quantification method of somatic mutations in normal tissues and their accumulation in pediatric patients with chemotherapy.
Proceedings of the National Academy of Sciences of the United States of America - 2 Aug 2022
Ueda Sho, Yamashita Satoshi, Nakajima Miho, Kumamoto Tadashi, Ogawa Chitose, Liu Yu-Yu, Yamada Harumi, Kubo Emi, Hattori Naoko, Takeshima Hideyuki, Wakabayashi Mika, Iida Naoko, Shiraishi Yuichi, Noguchi Masayuki, Sato Yukio, Ushijima Toshikazu
Abstract excerpt
Somatic mutations are accumulated in normal human tissues with aging and exposure to carcinogens. If we can accurately count any passenger mutations in any single DNA molecule, since their quantity is much larger than driver mutations, we can sensitively detect mutation accumulation in polyclonal normal tissues. Duplex sequencing, which tags both DNA strands in one DNA molecule, enables accurate count of such...
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