Article
Detection of low prevalence somatic mutations in solid tumors with ultra-deep targeted sequencing.
Genome biology - 20 Dec 2011
Harismendy Olivier, Schwab Richard B, Bao Lei, Olson Jeff, Rozenzhak Sophie, Kotsopoulos Steve K, Pond Stephanie, Crain Brian, Chee Mark S, Messer Karen, Link Darren R, Frazer Kelly A
Abstract excerpt
Ultra-deep targeted sequencing (UDT-Seq) can identify subclonal somatic mutations in tumor samples. Early assays' limited breadth and depth restrict their clinical utility. Here, we target 71 kb of mutational hotspots in 42 cancer genes. We present novel methods enhancing both laboratory workflow and mutation detection. We evaluate UDT-Seq true sensitivity and specificity (> 94% and > 99%, respectively) for low...
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