Article
Genotyping of Plasmodium falciparum to Assess Clone Composition in Parasite Cultures.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Broumou Ioanna, Plaza David Fernando, Färnert Anna
Abstract excerpt
Defining clone composition in Plasmodium falciparum cultures is key to verify that in vitro experiments are performed on the parasite line of interest. Genotyping of the highly polymorphic merozoite surface protein 2 gene (msp2) is a widely established method to define P. falciparum clones. Specific size variants from the two msp2 families (IC and FC27) can be used as "fingerprints" to identify individual clones...
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