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Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications.

Molecular and biochemical parasitology - 1 Oct 2005

Hunt Paul, Fawcett Richard, Carter Richard, Walliker David

Abstract excerpt

We have developed a rapid and simple method for determining accurately the proportions of alleles or individual malaria clones in a mixed infection. The technique uses a nested PCR reaction to amplify, from parasite mixtures, alleles of genes differing by single nucleotide polymorphisms, simultaneously, using common primers to non-polymorphic sequences. The mixed products are sequenced, and the heights of...

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PMID: 16061293
DOI: 10.1016/j.molbiopara.2005.05.014

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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