Article
Validation of microsatellite markers for use in genotyping polyclonal Plasmodium falciparum infections.
The American journal of tropical medicine and hygiene - 1 Nov 2006
Greenhouse Bryan, Myrick Alissa, Dokomajilar Christian, Woo Jonathan M, Carlson Elaine J, Rosenthal Philip J, Dorsey Grant
Abstract excerpt
Genotyping methods for Plasmodium falciparum drug efficacy trials have not been standardized and may fail to accurately distinguish recrudescence from new infection, especially in high transmission areas where polyclonal infections are common. We developed a simple method for genotyping using previously identified microsatellites and capillary electrophoresis, validated this method using mixtures of laboratory...
Topics
- Animals
- Genotype
- Humans
- Malaria, Falciparum
- Microsatellite Repeats
- Plasmodium falciparum
- Protozoan Proteins