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CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity

7 Jun 2022

Abstract excerpt

CRISPR-Cas12a (Cpf1) is a bacterial RNA-guided nuclease that cuts double-stranded DNA (dsDNA) at sites specified by a CRISPR RNA (crRNA) guide. Additional activities have been ascribed to this enzyme in vitro: site-specific (cis) single-stranded DNA (ssDNA) cleavage and indiscriminate (trans) degradation of ssDNA, RNA, and dsDNA after activation by a complementary target. The ability of Cas12a to cleave nucleic...

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PMID: 35670674
DOI: 10.1093/nar/gkac462

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