Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors.

Abstract excerpt

Site-specific recombinases (SSRs) are critical for achieving precise spatiotemporal control of engineered alleles. These enzymes play a key role in facilitating the deletion or inversion of loci flanked by recombination sites, resulting in the activation or repression of endogenous genes, selection markers or reporter elements. However, multiple recombination in complex alleles can be laborious. To address this,...

Topics

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• Alleles
• Blastocyst
• Dependovirus
• DNA Nucleotidyltransferases
• Gene Conversion