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Article

Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid.

Pathology oncology research : POR - 1 Jan 2022

Zaytseva Margarita, Usman Natalia, Salnikova Ekaterina, Sanakoeva Agunda, Valiakhmetova Andge, Chervova Almira, Papusha Ludmila, Novichkova Galina, Druy Alexander

Abstract excerpt

Cell-free DNA (cfDNA) in body fluids is invaluable for cancer diagnostics. Despite the impressive potential of liquid biopsies for the diagnostics of central nervous system (CNS) tumors, a number of challenges prevent introducing this approach into routine laboratory practice. In this study, we adopt a protocol for sensitive detection of the H3 K27M somatic variant in cerebrospinal fluid (CSF) by using digital...

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Identifiers

PMID: 35498161
DOI: 10.3389/pore.2022.1610024
PMCID: PMC9039021

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