Article
Efficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing.
Nature biotechnology - 23 Oct 2011
Myllykangas Samuel, Buenrostro Jason D, Natsoulis Georges, Bell John M, Ji Hanlee P
Abstract excerpt
We describe an approach for targeted genome resequencing, called oligonucleotide-selective sequencing (OS-Seq), in which we modify the immobilized lawn of oligonucleotide primers of a next-generation DNA sequencer to function as both a capture and sequencing substrate. We apply OS-Seq to resequence the exons of either 10 or 344 cancer genes from human DNA samples. In our assessment of capture performance, >87% of...
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