Article
Mitochondrial variant enrichment from high-throughput single-cell RNA sequencing resolves clonal populations.
Nature biotechnology - 1 Jul 2022
Miller Tyler E, Lareau Caleb A, Verga Julia A, DePasquale Erica A K, Liu Vincent, Ssozi Daniel, Sandor Katalin, Yin Yajie, Ludwig Leif S, El Farran Chadi A, Morgan Duncan M, Satpathy Ansuman T, Griffin Gabriel K, Lane Andrew A, Love J Christopher, Bernstein Bradley E, Sankaran Vijay G, van Galen Peter
Abstract excerpt
The combination of single-cell transcriptomics with mitochondrial DNA variant detection can be used to establish lineage relationships in primary human cells, but current methods are not scalable to interrogate complex tissues. Here, we combine common 3' single-cell RNA-sequencing protocols with mitochondrial transcriptome enrichment to increase coverage by more than 50-fold, enabling high-confidence mutation...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.