Article
N-terminal T4 lysozyme fusion facilitates crystallization of a G protein coupled receptor.
PloS one - 1 Jan 2012
Zou Yaozhong, Weis William I, Kobilka Brian K
Abstract excerpt
A highly crystallizable T4 lysozyme (T4L) was fused to the N-terminus of the β(2) adrenergic receptor (β(2)AR), a G-protein coupled receptor (GPCR) for catecholamines. We demonstrate that the N-terminal fused T4L is sufficiently rigid relative to the receptor to facilitate crystallogenesis without thermostabilizing mutations or the use of a stabilizing antibody, G protein, or protein fused to the 3rd...
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