Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns.

Abstract excerpt

Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools for precise genome editing in plants. Conventionally, such base editors are built upon the CRISPR-Cas9 systems where Cas9 nickases are used. To expand the base editing scope and minimize off-target effects, base editors derived from the CRISPR-Cas12a systems are desired. However, the use of deactivated Cas12a (dCas12a) in such base...

Topics

• Oryza
• Introns
• CRISPR-Cas Systems
• Gene Editing
• Mutation
• Base Sequence
• Plants, Genetically Modified