Article
CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles.
BMC biology - 7 Jan 2022
Mianné Joffrey, Nasri Amel, Van Chloé Nguyen, Bourguignon Chloé, Fieldès Mathieu, Ahmed Engi, Duthoit Christine, Martin Nicolas, Parrinello Hugues, Louis Anaïs, Iché Alexandra, Gayon Régis, Samain Florine, Lamouroux Lucille, Bouillé Pascale, Bourdin Arnaud, Assou Said, De Vos John
Abstract excerpt
BACKGROUND: The application of CRISPR/Cas9 technology in human induced pluripotent stem cells (hiPSC) holds tremendous potential for basic research and cell-based gene therapy. However, the fulfillment of these promises relies on the capacity to efficiently deliver exogenous nucleic acids and harness the repair mechanisms induced by the nuclease activity in order to knock-out or repair targeted genes. Moreover,...
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