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Article

Lineage recording in human cerebral organoids.

Nature methods - 1 Jan 2022

He Zhisong, Maynard Ashley, Jain Akanksha, Gerber Tobias, Petri Rebecca, Lin Hsiu-Chuan, Santel Malgorzata, Ly Kevin, Dupré Jean-Samuel, Sidow Leila, Sanchis Calleja Fatima, Jansen Sophie M J, Riesenberg Stephan, Camp J Gray, Treutlein Barbara

Abstract excerpt

Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enable highly resolved descriptions of cell states within these systems; however, approaches are needed to directly measure lineage relationships. Here we establish iTracer, a lineage recorder that combines reporter barcodes with inducible CRISPR-Cas9 scarring and is compatible with...

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Identifiers

PMID: 34969984
DOI: 10.1038/s41592-021-01344-8
PMCID: PMC8748197

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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