Article
De novo screening of disease-resistant genes from the chromosome-level genome of rare minnow using CRISPR-cas9 random mutation.
GigaScience - 19 Nov 2021
Huang Rong, Shi Mijuan, Luo Lifei, Yang Cheng, Ou Mi, Zhang Wanting, Liao Lanjie, Li Yongming, Xia Xiao-Qin, Zhu Zuoyan, Wang Yaping
Abstract excerpt
BACKGROUND: Mutants are important for the discovery of functional genes and creation of germplasm resources. Mutant acquisition depends on the efficiency of mutation technology and screening methods. CRISPR-Cas9 technology is an efficient gene editing technology mainly used for editing a few genes or target sites, which has not been applied for the construction of random mutant libraries and for the de novo...
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