Article
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC biotechnology - 22 May 2015
Nakagawa Yoshiko, Sakuma Tetsushi, Sakamoto Takuya, Ohmuraya Masaki, Nakagata Naomi, Yamamoto Takashi
Abstract excerpt
BACKGROUND: Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing permits the rapid production of genetically engineered mice. To make the most of this innovative technology, a streamlined procedure is needed for the robust construction of CRISPR/Cas9 vectors, the efficient preparation of mouse oocytes, and refined genotyping strategies....
Topics
- Animals
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- DNA
- Fertilization in Vitro
- Mice
- Mice, Knockout
- Microinjections
- Mutagenesis
- Mutation
- Oocytes