Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.

BMC biotechnology - 22 May 2015

Nakagawa Yoshiko, Sakuma Tetsushi, Sakamoto Takuya, Ohmuraya Masaki, Nakagata Naomi, Yamamoto Takashi

Abstract excerpt

BACKGROUND: Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing permits the rapid production of genetically engineered mice. To make the most of this innovative technology, a streamlined procedure is needed for the robust construction of CRISPR/Cas9 vectors, the efficient preparation of mouse oocytes, and refined genotyping strategies....

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Associated Proteins
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Fertilization in Vitro

Share to Mice, Knockout

Share to Microinjections

Share to Mutagenesis

Share to Oocytes

Share to Zygote

Source

Open in PubMed Open full text

Identifiers

PMID: 25997509
DOI: 10.1186/s12896-015-0144-x
PMCID: PMC4440308

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.PMID 25997509Read ↗

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice.Semantic proximity Read ↗

Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein.Semantic proximity Read ↗

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote.Semantic proximity Read ↗

A non-inheritable maternal Cas9-based multiple-gene editing system in mice.Semantic proximity Read ↗

Generation of Genetically Modified Mice through the Microinjection of Oocytes.Semantic proximity Read ↗

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering.Semantic proximity Read ↗

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.Semantic proximity Read ↗

Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system.Semantic proximity Read ↗

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes.Semantic proximity Read ↗

Recovering a Targeted Mutation in Mice from Embryonic Stem Cell Chimeras or CRISPR-Cas Founders.Semantic proximity Read ↗

A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders Using the CRISPR-Cas System.Semantic proximity Read ↗

Genome editing for the reproduction and remedy of human diseases in mice.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.