Article
Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Jul 2016
Shigeta Mitsuki, Sakane Yuto, Iida Midori, Suzuki Miyuki, Kashiwagi Keiko, Kashiwagi Akihiko, Fujii Satoshi, Yamamoto Takashi, Suzuki Ken-Ichi T
Abstract excerpt
Recent advances in genome editing using programmable nucleases, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system, have facilitated reverse genetics in Xenopus tropicalis. To establish a practical workflow for analyzing genes of interest using CRISPR-Cas9, we examined various...
Topics
- Animals
- CRISPR-Cas Systems
- Embryo, Nonmammalian
- Embryonic Development
- Gene Editing
- Gene Targeting
- Genetic Engineering
- Mutation
- Phenotype
- RNA, Messenger