Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Comprehensive transposon mutant library of Pseudomonas aeruginosa.

Proceedings of the National Academy of Sciences of the United States of America - 25 Nov 2003

Jacobs Michael A, Alwood Ashley, Thaipisuttikul Iyarit, Spencer David, Haugen Eric, Ernst Stephen, Will Oliver, Kaul Rajinder, Raymond Christopher, Levy Ruth, Chun-Rong Liu, Guenthner Donald, Bovee Donald, Olson Maynard V, Manoil Colin

Abstract excerpt

We have developed technologies for creating saturating libraries of sequence-defined transposon insertion mutants in which each strain is maintained. Phenotypic analysis of such libraries should provide a virtually complete identification of nonessential genes required for any process for which a suitable screen can be devised. The approach was applied to Pseudomonas aeruginosa, an opportunistic pathogen with a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Escherichia coli
Share to Gene Library

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genes, Bacterial

Share to Mutagenesis, Insertional

Share to Mutation

Share to Phenotype

Share to Pseudomonas aeruginosa

Share to Species Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 14617778
DOI: 10.1073/pnas.2036282100
PMCID: PMC283593

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Comprehensive transposon mutant library of Pseudomonas aeruginosa.PMID 14617778Read ↗

Sequence-verified two-allele transposon mutant library for Pseudomonas aeruginosa PAO1.Semantic proximity Read ↗

Transposon insertion site sequencing (TIS) of Pseudomonas aeruginosa.Semantic proximity Read ↗

An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants.Semantic proximity Read ↗

Transposon mutagenesis.Semantic proximity Read ↗

Screening of the PA14NR Transposon Mutant Library Identifies Genes Involved in Resistance to Bacteriophage Infection in Pseudomomas aeruginosa.Semantic proximity Read ↗

In vivo functional genomics of Pseudomonas aeruginosa for high-throughput screening of new virulence factors and antibacterial targets.Semantic proximity Read ↗

Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon-sequencing libraries.Semantic proximity Read ↗

Targeted genetic screening in bacteria with a Cas12k-guided transposase.Semantic proximity Read ↗

Massively parallel mutant selection identifies genetic determinants of <i>Pseudomonas aeruginosa</i> colonization of <i>Drosophila melanogaster</i>Semantic proximity Read ↗

Sequence-Defined Transposon Mutant Library of Burkholderia thailandensisSemantic proximity Read ↗

A toolkit for transposon libraries and functional genomics in intestinal BacteroidalesSemantic proximity Read ↗

Reconstruction of the metabolic network of Pseudomonas aeruginosa to interrogate virulence factor synthesis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.